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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has been observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in promoting cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been explained. Two supplemental splice variants have been noted but detailed nucleotide sequence facts isn't but obtainable.

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Blocking peptides are peptides that bind specifically to the goal antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.

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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to ensure the very best benefits are...

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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best success are...

Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining through the blocked antibody vs . the antibody by yourself, you can see which staining is particular.

Target Information Cyclin E belongs to the extremely conserved cyclin household, whose customers are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of every mitotic occasion. Cyclin E kinds a complex with and features for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and it is degraded as cells development by way of S phase.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...

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